Exonuclease I (E.coli) from New England Biolabs

Product Specs
ItemExonuclease I (E.coli)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0293L
Quantity15000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [³H]-DNA.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dbh+ catecholaminergic cardiomyocytes contribute to the structure and function of the cardiac conduction system in murine heartNature CommunicationsNovember 28, 2023Tianyi Sun, Alexander Grassam-Rowe, Zhaoli Pu, Yangpeng Li, Huiying Ren, Yanru An, Xinyu Guo, Wei Hu, Ying Liu, Yuqing Zheng, et al.min. After washing with 0.1× SSC buffer for two times, the chips were treated with Exonuclease I (NEB, M0293L) at 37 °C incubator for 1 h. After washing once with 0.1x SSC buffer, cDNA was amplified using KAPA HiFi

(2) Clinically relevant antibiotic resistance genes are linked to a limited set of taxa within gut microbiome worldwideNature CommunicationsNovember 14, 2023Peter J. Diebold, Matthew W. Rhee, Qiaojuan Shi, Nguyen Vinh Trung, Fayaz Umrani, Sheraz Ahmed, Vandana Kulkarni, Prasad Deshpande, Mallika Alexander, Ngo Thi Hoa, et al.72 °C for 5 minutes. Duplicate reactions were pooled and treated with heat-labile Exonuclease I (NEB M0293L) to degrade the primers (10U ExoI per reaction, incubated at 37 °C for 25 min followed by 5 min at 80

(3) Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammationImmunityOctober 10, 2023Kami Pekayvaz, Christoph Gold, Parandis Hoseinpour, Anouk Engel, Alejandro Martinez-Navarro, Luke Eivers, Raffaele Coletti, Markus Joppich, Flávio Dionísio, Rainer Kaiser, et al.cleaned using cleanup beads (1:1 beads/sample ratio). Remaining primers were digested with Exonuclease I (M0293L, NEB) following cleanup at 37 °C for 20 minutes followed by 80 °C for 10 minutes. The Exonuclease I

(4) SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stressbioRxivJuly 17, 2023Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovicto ligate tags during split-and-pool barcoding. Excess primer is digested with Exonuclease I (NEB, #M0293L) at 37°C for 15 minutes. Split-and-pool barcoding to identify RNA-protein interactions Split-and-pool

(5) Whole-genome transcriptome and DNA methylation dynamics of pre-implantation embryos reveal progression of embryonic genome activation in buffaloesJournal of Animal Science and BiotechnologyJuly 11, 2023Penghui Fu, Du Zhang, Chunyan Yang, Xiang Yuan, Xier Luo, Haiying Zheng, Yanfei Deng, Qingyou Liu, Kuiqing Cui, Fei Gao, et al.Buffer (P7010-HC-L, Enzymatics, Beverly, MA, USA). Subsequently, DNA was incubated with exonuclease I (M0293L, NEB, Ipswich, England) for 1 h at 37 °C and purified with Agencourt Ampure XP (A63881, Beckman Coulter,

(6) Systematic screening identifies ABCG2 as critical factor underlying synergy of kinase inhibitors with transcriptional CDK inhibitorsBreast Cancer ResearchMay 5, 2023Vera E. van der Noord, Wanda van der Stel, Gijs Louwerens, Danielle Verhoeven, Hendrik J. Kuiken, Cor Lieftink, Melanie Grandits, Gerhard F. Ecker, Roderick L. Beijersbergen, Peter Bouwman, et al.reactions composed of 44 µL 10 mM Tris–HCl (pH 8), 5 µL 10X Exonuclease buffer, and 1 µL Exonuclease I (M0293L, New England Biolabs), at 37 °C for 1 h. Beads were washed 3 times with 10 mM Tris–HCl (pH 8) and resuspended

(7) Primary role of type I interferons for the induction of functionally optimal antigen-specific CD8(+) T cells in HIV infectionEBioMedicineMay 1, 2023Cabral-Piccin MP, Papagno L, Lahaye X, Perdomo-Celis F, Volant S, White E, Monceaux V, Llewellyn-Lacey S, Fromentin R, Price DA, et al.at 96 °C and 4 min at 60 °C. Samples were then treated with Exonuclease I (New England BioLabs, CAT#M0293L) for 30 min at 37 °C and 15 min at 80 °C, diluted 5-fold in DNA Suspension Buffer (Teknova, CAT#T0221),

(8) Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokinesScience advancesApril 21, 2023Licht-Murava A, Meadows SM, Palaguachi F, Song SC, Jackvony S, Bram Y, Zhou C, Schwartz RE, Froemke RC, Orr AL, et al.Grandmaster mix (TATAA Biocenter, Sweden, #TA05) before exonuclease I treatment (New England Biolabs, #M0293L). Preamplified cDNA was diluted at least fivefold with nuclease-free water and mixed with SsoFast EvaGreen

(9) Conjugative IncC Plasmid Entry Triggers the SOS Response and Promotes Effective Transfer of the Integrative Antibiotic Resistance Element SGI1Microbiology SpectrumFebruary 14, 2023Marine C. Pons, Karine Praud, Sandra Da Re, Axel Cloeckaert, Benoît Doubletmin at 60°C and a final hold at 4°C. Samples were treated with exonuclease I to removed primers (NEB; M0293L) and diluted 20-fold in Tris-EDTA. Preamplified cDNA samples and primer pairs were loaded on the Fluidigm

(10) A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociationMolecular Systems BiologyFebruary 10, 2023Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp Junker#E2040S) for 16 h at 37°C. Residual primers were removed by adding 3 μl Exo I (New England Biolabs #M0293L) and 3 μl rSAP (New England Biolabs #M0371L) to 16 μl of aRNA. Afterwards we fragmented the aRNA by

(11) Temporal and sequential transcriptional dynamics define lineage shifts in corticogenesisThe EMBO JournalDecember 15, 2022Tanzila Mukhtar, Jeremie Breda, Manal A Adam, Marcelo Boareto, Pascal Grobecker, Zahra Karimaddini, Alice Grison, Katja Eschbach, Ramakrishnan Chandrasekhar, Swen Vermeul, et al.Mix,Fluidigm,100‐6300 Human Brain Reference RNA,Life Technologies,AM6050 Exonuclease I,New England Biolabs,M0293L C1 Single Cell Auto Prep Array for mRNA Seq Multipack—contains: Module 1 Single Cell Auto Prep KitModule

(12) Interpreting and de-noising genetically engineered barcodes in a DNA virusPLOS Computational BiologyNovember 22, 2022Sylvain Blois, Benjamin M. Goetz, James J. Bull, Christopher S. Sullivanshown in S3 Fig). Briefly, enrichment PCR products were digested with 20U of ExoI (New England Biolabs, M0293L) at 37°C for 30 minutes to remove residual primers and heat inactivated at 80°C for 20 min before proceeding

(13) Spatially resolved gene regulatory and disease-related vulnerability map of the adult Macaque cortexNat CommunNovember 8, 2022Ying Lei, Mengnan Cheng, Zihao Li, Zhenkun Zhuang, Liang Wu, Yunong Sun, Lei Han, Zhihao Huang, Yuzhou Wang, Zifei Wang, et al.100 mM NaCl, and 0.5% SDS) at 37 °C for 30 min. The chips were then treated with Exonuclease I (NEB, M0293L) at 37 °C for 1 h and washed twice with the wash buffer. The resulting first strand cDNAs on the chips

(14) Repurposing the cardiac glycoside digoxin to stimulate myelin regeneration in chemically-induced and immune-mediated mouse models of multiple sclerosisGliaOctober 1, 2022Titus HE, Xu H, Robinson AP, Patel PA, Chen Y, Fantini D, Eaton V, Karl M, Garrison ED, Rose IVL, et al.Mastermix, Applied Biosystems 44‐885‐93), removal of excess primers (Exonuclease I, New England BioLabs M0293L), and dilution of sample. Five microliters of sample mix containing pre‐amplified cDNA and amplification

(15) High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoidsSTAR ProtocolsSeptember 16, 2022Santiago Cerrizuela, Oguzhan Kaya, Lukas P.M. Kremer, Andrea Sarvari, Tobias Ellinger, Jannes Straub, Jan Brunken, Andrés Sanz-Morejón, Aylin Korkmaz, Ana Martín-VillalbaResearch,Cat#D5002-3 M-Desulphonation buffer,Zymo Research,Cat#D5001-5 Klenow (3′-5′ exo-),Biozym,Cat#280310 Exonuclease I,NEB,Cat#M0293L Recombinant RNAse inhibitor,Takara Bio,Cat#2313A Agencourt AMPure XP beads,Beckman Coulter,Cat#A63881 Maxima

(16) Cell landscape of larval and adult Xenopus laevis at single-cell resolutionNature CommunicationsJuly 25, 2022Yuan Liao et al.exonuclease I mix [1× exonuclease I buffer (Cat. No. B0293S, NEB), and 1 U/µl exonuclease I (Cat. No. M0293L, NEB)] and incubated at 37 °C for 30 min with mixing on a rotary mixer to remove single-stranded oligonucleotides

(17) Epigenetic modifier SMCHD1 maintains a normal pool of long-term hematopoietic stem cellsiScienceJuly 15, 2022Kinkel SA, Liu J, Beck T, Breslin KA, Iminitoff M, Hickey P, Blewitt ME.mL Eppendorf LoBind tube (Eppendorf, 0030108051). Exonuclease treatment and clean-up Exo I (NEB - M0293L; 20 U/ul) was added to the pooled sample at a final concentration 1 U/ul concentration and gently mixed

(18) DRUG-seq Provides Unbiased Biological Activity Readouts for Neuroscience Drug DiscoveryACS Chemical BiologyJune 17, 2022Jingyao Li et al.treatment was performed on all samples by adding 4 μL of ExoI buffer and 4 μL of ExoI (New England Biolabs, M0293L). Samples were incubated at 37 C for 30 min, heat inactivated at 85 C for 15 min, and held at 4 C. cDNA

(19) A Cellular Resolution Spatial Transcriptomic Landscape of the Medial Structures in Postnatal Mouse BrainFrontiers in Cell and Developmental BiologyMay 17, 2022Mengnan Cheng et al.EDTA, 100 mM NaCl, and 0.5% SDS) at 37°C for 30 min. The chip was then treated with exonuclease I (NEB, M0293L) for 1 h at 37°C and washed twice with the wash buffer. The resulting first-strand cDNAs were amplified

(20) Identification of Key Factors for Anoxic Survival of B. cenocepacia H111International Journal of Molecular SciencesApril 20, 2022Sarah Paszti, Alessandra Vitale, Yilei Liu, Rubina Braunwalder, Ratchara Kalawong, Olivier Biner, Gabriella Pessi, Leo EberlBamHI site within the transposon. Circularization followed by exonuclease treatment with ExoI (NEB, M0293L), T7 Gene 6 Exo (NEB, M0263S) and Lambda Exo (NEB, M0262S) occurred with size selected DNA fragments.

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